Modulation of extracytoplasmic function (ECF) sigma factor promoter selectivity by spacer region sequence

Modulation of extracytoplasmic function (ECF) sigma factor promoter selectivity by spacer region sequence

The ability of bacteria to adapt to stress depends on the conditional expression of specific sets of genes. Bacillus subtilis encodes seven extracytoplasmic function (ECF) sigma (σ) factors that regulate functions important for survival under conditions eliciting cell envelope stress. Of these, four have been studied in detail: σM, σW, σX and σV. These four σ factors recognize overlapping sets ...

The extracytoplasmic function (ECF) sigma factors.

Bacterial sigma (sigma) factors are an essential component of RNA polymerase and determine promoter selectivity. The substitution of one sigma factor for another can redirect some or all of the RNA polymerase in a cell to activate the transcription of genes that would otherwise be silent. As a class, alternative sigma factors play key roles in coordinating gene transcription during various stre...

A Novel Extracytoplasmic Function (ECF) Sigma Factor Regulates Virulence in Pseudomonas aeruginosa

Next to the two-component and quorum sensing systems, cell-surface signaling (CSS) has been recently identified as an important regulatory system in Pseudomonas aeruginosa. CSS systems sense signals from outside the cell and transmit them into the cytoplasm. They generally consist of a TonB-dependent outer membrane receptor, a sigma factor regulator (or anti-sigma factor) in the cytoplasmic mem...

Two Extracytoplasmic Function Sigma Subunits, s and s, of Escherichia coli: Promoter Selectivity and Intracellular Levels

The promoter selectivity of two extracytoplasmic function (ECF) subfamily s subunits, s (s) and s (s), of Escherichia coli RNA polymerase was analyzed by using an in vitro transcription system and various promoters. The Es holoenzyme recognized only the known cognate promoters, rpoEP2, rpoHP3, and degP, and the Es recognized only one known cognate promoter, fecA. The strict promoter recognition...

Knock - out of extracytoplasmic function sigma factor ECF - 10 affects stress resistance 1 and biofilm formation in Pseudomonas putida